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BBM medium

Authors: Bold 1949, Bischoff and Bold 1963

Useful for many algae, especially chlorococcalea algae, volvocalean algae (e.g.Chlamydomonas), filamentous green alga Klebsormidim flaccidum, xanthophycean alga Heterococcus endolithicus etc. (Andersen, 2005).

Into 900 ml of dH2O add 10 ml of first six stock solutions. Add 1 ml of rest solutions. Autoclave. The final pH should be 6,6 

Component

Stock Solution

(g.l-1 dH2O)

Quantity Used

(to 1 litre)

NaNO3 25,00 10 ml
CaCl2.2H2O 2,50 10 ml
MgSO4.7H2O 7,50 10 ml
K2HPO4 7,50 10 ml
KH2PO4 17,50 10 ml
NaCl 2,50 10  ml
EDTA solution see following recipe 1 ml
Acidifed iron solution see following recipe 1 ml
H3BO3 11,42 g 1 ml
Trace metals solution see following recipe 1 ml

EDTA solution 

Component

Quantity Used

(to 1 litre)

EDTA 50,00 g
KOH 31,00 g 

Acidified Iron Solution

Component

Quantity Used

(to 100 ml)

FeSO4.7H2O 0,498 g
H2SO(96%) 0,1 ml

Trace metals solution 

Component

Quantity Used

(to 1 litre)

ZnSO4.7H2O 8,82 g
MnCl2.4H2O 1,44 g
MoO3 0,71 g 
CuSO4.5H2O 1,57 g 
Co(NO3)2.6H2O 0,49 g

Literature:

  • ANDERSEN, R.A. [ed.] 2005Algal culturing techniques. Elsevier, Amsterdam, 578 pp. 
  • BISCHOFF, H.W., and BOLD, H.C. 1963.Phycological Studies IV. Some Soil Algae From Enchanted Rock and Related Algal Specie. University of Texas, Austin, 6318: 1 - 95.
  • BOLD, H.C. 1949. The morphology of Chlamydomonas chlamydogama sp. nov.. Bull. Torrey Bot. Club. 76: 101 - 108. 
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