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Brackish water

 
Component

Stock Solution

(g to100 ml dH2O)

Quantity Used

(to 1 litre)

KNO3 1,00  20 ml
K2HPO4 0,1 g 20 ml
MgSO4.7H2O 0,1 g 20 ml
Soil extract see following recipe 30 ml
Trace metal solution see following recipe 5 ml
dH2O - 450 ml
Artificial seawater see following recipe 450 ml

Soil extract

1/3 of 6 litre flask fill with garden or leaf soil (not too great humus or clay content), and deionized water until it stands 5cm above the soil. Sterilize by heating in a steamer one hour, twice in a 24h interval. Separate the decanted extract by centrifugation. Fill into small containers, autoclave 20 minutes/1 atmosphere; store in refrigerator.

Trace metals solution 

Component

Quantity Used

(to 1 litre)

ZnSO4.7H2O 8,82 g
MnCl2.4H2O 1,44 g
MoO3 0,71 g 
CuSO4.5H2O 1,57 g 
Co(NO3)2.6H2O 0,49 g

Artificial seawater 

Component

Quantity Used

(g.l-1 dH2O)

NaCl 60,0 g
MgSO4.7H2O 10,0 g 
KCl 1,5 g
CaSO4.2H2O 2,0 g

 

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