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Galdieria medium - modified

authors: Gross, W. & Schnarrenberger, 1995

Dissolve chemicals in 500 ml of distilled water. Add 2,07 ml of Fe-EDTA solution and 2,00 ml trace metal solution. Make up to 1000 ml. Autoclave.

Component

Quantity Used

(to 1 litre)

(NH4)2SO4 1,5 g
MgSO4.7H2O 0,3 g
KH2PO4 0,3 g 
CaCl2.2H2O 0,02 g
Fe-EDTA (see following recipe) 2,07 ml
Trace metals solution (see following recipe) 2,00 ml 

Fe-EDTA solution 

To 50 ml od dH2O add following component and fill in with dH2O do 100 ml. 

Component

Quantity Used

KON (1 N solution) 5,4 ml
FeSO4.7H2O 0,5 ml
EDTA (no Na-EDTA!) 0,52 g 

Trace metals solution 

Component

Quantity Used

(to 100 ml)

H3BO3 0,31 g
MnSO4.4H2O 0,223 g
Na2WO4.2H2O 0,003 g
(NH4)6Mo7O24.4H2O 0,0088 g
KBr 0,0119 g
KI 0,0083 g
ZnSO4.7H2O 0,0287 g
Cd(NO3)2.4H2O 0,0154 g
Co(NO3)2.6H2O 0,0146 g
CuSO4.5H2O 0,0125 g
NiSO4(NH4)2SO4.6H2O 0,0198 g
Cr(NO3)2.7H2O 0,0037 g
V2O4(SO4)3.16H2O 0,0035 g
Al2(SO4)3K2SO4.24 H2O 0,0474 g

Literautre: 

  • Gross, W. & Schnarrenberger, C. 1995. Heterotrophic Growth of Two Strains of the Acido-Thermophilic Red Alga Galdieria sulphuraria. Plant Cell. Physiol. 36(4): 633 - 638.  
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