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Medium for non-seed plants

Authors: Baslerová & Dvořáková 1962

Liverworts, ferns and duckweeds are maintained in 250 ml Erlenmayer's flasks on agar medium in the air conditioned room at 12-15°C. They are irradiated similarly as other strains (see above).

Into 900 ml of dH2O add quantity of stock solutions (see belowe) fill in 1 litre with dH2O. Autoclave. 

Component

Stock Solution

(g.l-1 dH2O)

Mosses (M)

Quantity Used

(to 1 litre)

Liverworts & ferns 
(JKL)

Quantity Used

(to 1 litre)

NH4NO3 80,00 1.5 ml 2.5 ml
KH2PO4 136,00 0.5 ml 0.75 ml
MgSO4.7H2O 120,00 0.5 ml 0.4 ml
CaCl2.2H2O 110,00 0.2 ml 1,0 ml
FeCl3.6H2O 16.20 0.2 ml 0.16 ml

Literature:

  • Baslerová, M. & Dvořáková, J. 1962. Algarum, Hepaticarum Muscurumque in cultiris collectio. NCSAV, Praha, 59 pp. 
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