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Spirulina medium

Authors: Aiba and Ogawa 1977, Schlösser 1994

For culturing Spirulina. 

Prepare Solutions I and II. Autoclave separately and cool; asepticilly combine the two solutions. Aseptically add 1 ml of cyanocobalamin (B12) solution. 

Component

Stock Solution

(g.l-1 dH2O)

Quantity Used

(to 500 ml)

Solution I. (500 ml)
NaHCO3 - 13,61 g
Na2CO3 - 4,03 g
K2HPO4 - 0,50 g 
Solution II. (500 ml)
NaNO3 - 2,5 g
K2SO4 - 1,0 g
NaCl - 1,0 g
MgSO4.7H2O - 0,2 g 
CaCl2.2H2O - 0,04 g
FeSO4.7H2O - 0,01 g
Na2EDTA.2H2O - 0,08 g
Trace metals solution see following recipe 5 ml
Vitamins solution see following recipe 1 ml

Trace metals solution 

Component

Stock Solution

(g.l-1 dH2O)

Quantity Used

(to 1 litre)

Na2EDTA.2H2O - 0,5 g
FeSO4.7H2O - 0,7 g
ZnSO4.7H2O 1,0 1 ml
MnSO4.7H2O 2,0 1 ml
H3BO3 10,0 1 ml
Co(NO3)2.6H2O 1,0 1 ml
Na2MoO4.2H2O 1,0 1 ml
CuSO4.5H2O 0,005 1 ml

Vitamin Solution

Dissolve the cyanocobalamin in 1 liter dH2O. Filter-sterilize and store frozen. 

Component

Quantity Used

(to 1 litre)

Cyanocobalamin (vit. B12) 5 mg

Literauture

  • AIBA, S., and OGAWA, T. 1977. Assessment of growth yield of a blue-green alga:Spirulina platensis in axenic and continuous cultur. J. Gen. Microbiol. 102: 179 - 182. 
  • ANDERSEN, R.A. [ed.] 2005Algal culturing techniques. Elsevier, Amsterdam, 578 pp. 
  • SCHLÖSSER, U.G. 1994. SAG-Sammlung von Algenkulturen at the University of Göttingen Catalogue of Strains 1994. Bot. Acta 107: 111 - 186.
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