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WC

authors: Guillard & Lorenzen, 1972

For planktonic algae and cyanobacteria.

Combine 1 ml of stock solution and add TRIS buffer. Autoclave. Add sterille vitamin solution do cool medium.

Component Stock Solution (g.l-1 dH2O) Quantiny Used (to 1 litre)
CaCl2.2H2O 36,8 g 1 ml
MgSO4.7H2O 37,0 g 1 ml
NaHCO3 12,6 g 1 ml
K2HPO4.3H2O 5,7 g 1 ml
NaNO3 42,5 g 1 ml
Na2SiO3.5H2O 21, 2 g 1 ml
trace metal solution see bellow 1 ml
vitamins solution see bellow 1 ml
TRIS - 0,115 g

Trace metals solution

Component Quantity Used (to 1 litre)
Na2EDTA 4,360 g
FeCl3.6H2O 3,150 g
CuSO4.5H2O 0,010 g
ZnSO4.7H2O 0,022 g
CoCl2.6H2O 0,010 g
MnCl2.4H2O 0,180 g
Na2MoO4.2H2O 0,006 g
H3BO3 1,000 g

Vitamins solution

Sterile by filtration

Component Quantity used (to 1 litre)
thiamin 0,1 g
biotin 0,0005 g
cyanokabalamin 0,0005 g

Literature:

  • GUILLARD, R.R.L., LORENZEN, C.J. (1972): Yellow-green algae with chlorophyllide C. Journal of Phycology 8: 10-14
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