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Gorham's solution

authors: Koeman & Hoek 1980

In 1000 ml distilled water dissolve the following chemicals and add 2 ml of soil extract .


Quantity Used

(to 1 litre)

NaNO3 160 mg
K2HPO4 17,5 mg
TRIS buffer 200 mg
MgSO4.7H2O 153,6 mg
CuSO4.5H2O 0,0156 mg
CaCl2 35 mg
Na2SiO3 11,63 mg
Na2CO3 20 mg
Fe-citrate 3 mg
Citric acid 3 mg
Na-EDTA (Chelaton III) 600 mg
Na2MoO4.2H2O 0,00705 mg
ZnSO4.7H2O 0,0392 mg
CoCl2 0,0183 mg
MnCl2.4H2O 0,283 mg

Soil extract

1/3 of 6 litre flask fill with garden or leaf soil (not too great humus or clay content), and deionized water until it stands 5cm above the soil. Sterilize by heating in a steamer one hour, twice in a 24h interval. Separate the decanted extract by centrifugation. Fill into small containers, autoclave 20 minutes/1 atmosphere; store in refrigerator.


  • KOEMAN,R.P.T. & HOEK,C. van den (1980): The taxonomy of Ulva (Chlorophyceae) in the Netherland. - Brit.Phycol.J. 16: 9-53.
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