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HS medium for Chlamydomonas

author: Sueoka 1960

Into 500 ml of dH2O add quantity of stock solutions (see belowe) fill in 990 mililitre with dH2O. Autoclave. After cooling add 10 ml od stock solution no. 5. 

Component

Stock Solution

(g.l-1 dH2O)

Quantity Used

(to 1000 ml)

Stock stolution 1. 
NH4Cl 50,00 10 ml
CaCl2.2H2O 2,00
Stock solution 2.
MgSO4.7H2O 20,00 10 ml
FeNa-EDTA 1,84
Stock solution 3.
Na2MoO4.2H2O 5,00 (or 0,5 g to 100 ml dH2O) 0,5 ml
Stock solution 4. 
Trace metals solution see following recipe 1 ml
Stock solution 5. - add after autoclaving!
KH2PO4 72,00  5 ml
K2HPO4 134,00

Trace metals solution 

Component

Quantity Used

(to 1 litre)

H3BO3 3,086 g
MnSO4.4H2O 1,180 g
CoSO4.7H2O 1,404 g
CuSO4.5H2O 1,244 g
ZnSO4.7H2O 1,430 g
(NH4)6Mo7O24.4H2O 1,840 g

Literature:

  • Sueoka, N. 1960. MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI. PNAS 46(1): 83-91.
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