Dissolve the chemicals in 500 ml of distilled water, add 1 ml of trace metals solution and make up to 1000 ml. Autoclave.
| Component |
Quantity Used (to 1 litre) |
|---|---|
| KCl | 1,0 g |
| NaHCO3 | 16,8 g |
| K2HPO4 | 0,5 g |
| NaNO3 | 2,5 g |
| K2SO4 | 1,0 g |
| NaCl | 30,0 g |
| MgSO4.7H2O | 0,18 g |
| FeSO4.7H2O | 0,018 g |
| EDTA | 0,08 g |
| Trace metal solution | 1 ml |
Trace metals solution
| Component |
Stock Solution (g.l-1 dH2O) |
Quantity Used (to 1 litre) |
|---|---|---|
| H3BO3 | - | 2,860 g |
| MnCl2.4H2O | - | 1,810 g |
| ZnSO4.7H2O | - | 0,220 g |
| CuSO4.5H2O | 79,0 | 1 ml |
| Na2MoO4.2H2O | - | 0,391 g |
| Co(NO3)2.6H2O | 49,4 | 1 ml |