HS medium for Chlamydomonas
Author: Sueoka, 1960
To 500 mL of dH₂O, add the appropriate volumes of stock solutions 1–4 . Bring the volume to 990 mL with dH₂O and autoclave. After cooling, add the appropriate volume of stock solution 5 aseptically.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
Stock stolution 1. |
||
| NH4Cl | 50,00 | 10 ml |
| CaCl2.2H2O | 2,00 | |
Stock solution 2. |
||
| MgSO4.7H2O | 20,00 | 10 ml |
| FeNa-EDTA | 1,84 | |
Stock solution 3. |
||
| Na2MoO4.2H2O | 5,00 (or 0,5 g to 100 ml dH2O) | 0,5 ml |
Stock solution 4. |
||
| Trace metals solution | see following recipe | 1 ml |
Stock solution 5. |
||
| Add after autoclaving! | ||
| KH2PO4 | 72,00 | 5 ml |
| K2HPO4 | 134,00 | |
Trace metals solution
| Component |
Quantity Used |
|---|---|
| H3BO3 | 3,086 g |
| MnSO4.4H2O | 1,180 g |
| CoSO4.7H2O | 1,404 g |
| CuSO4.5H2O | 1,244 g |
| ZnSO4.7H2O | 1,430 g |
| (NH4)6Mo7O24.4H2O | 1,840 g |
Literature:
- Sueoka, N. (1960). Mitotic replication of deoxyribonucleic acid in Chlamydomonas reinhardi. Proceedings of the National Academy of Sciences, 46(1), 83-91.