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CCALA Culture Collection
of Autotrophic Organisms

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Culture Collection of Autotrophic Organisms (CCALA)
Experimental Garden and Gene Pool Collections Třeboň
Institute of Botany of the CAS, v. v. i.
ccala@ibot.cas.cz

Jaworski’s Medium

Author: Schlösser 1982

For freshwater algae.

Dissolve stock solutions 1 - 9 (1 ml each) in 1 litre of distilled water.

Component	Stock Solutiong.l-1 dH2O	Quantity Usedto 1 litre
Ca(NO₃)₂.4H₂O	20,0	1 ml
KH₂PO₄	12,4	1 ml
MgSO₄.7H₂O	50,0	1 ml
NaHCO₃	15,9	1 ml
EDTA solution	see following recipe	1 ml
Trace metals solution	see following recipe	1 ml
NaNO₃	80,0	1 ml
Na₂HPO₄.12H₂O	36,0	1 ml
Vitamin solution	see following recipe	1 ml

EDTA-solution

Component	Quantity Usedto 200 ml
FeNa-EDTA	0,45 g
Na₂EDTA	0,45 g

Trace metals solution

Component	Quantity Usedto 200 ml
H₃BO₃	0,496 g
MnCl₂.4H₂O	0,278 g
(NH₄)₆Mo₇O₂₄.4H₂O	0,20 g

Vitamins solution

Component	Quantity Usedto 200 ml
cyanocobalamin	0,008 g
thiamine HCl	0,008 g
biotin	0,008 g

Literature:

ANDERSEN, R.A. [ed.] 2005. Algal culturing techniques. Elsevier, Amsterdam, 578 pp.
SCHLÖSSER, U.G., 1982. Sammlung von Algenkulturen. - Ber.Dt.bot.Ges. 95: 181-276.