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Jaworski’s Medium

Author: Schlösser 1982

For freshwater algae.

Dissolve stock solutions 1 - 9 (1 ml each) in 1 litre of distilled water.

 

Component

Stock Solutiong.l-1 dH2O

Quantity Usedto 1 litre
Ca(NO3)2.4H2O 20,0 1 ml
KH2PO4 12,4 1 ml 
MgSO4.7H2O 50,0 1 ml
NaHCO3 15,9 1 ml
EDTA solution see following recipe 1 ml
Trace metals solution see following recipe 1 ml
NaNO3 80,0 1 ml
Na2HPO4.12H2O 36,0 1 ml
Vitamin solution see following recipe 1 ml 

EDTA-solution

Component

Quantity Usedto 200 ml
FeNa-EDTA 0,45 g
Na2EDTA 0,45 g 

Trace metals solution

Component

Quantity Usedto 200 ml
H3BO3 0,496 g 
MnCl2.4H2O 0,278 g
(NH4)6Mo7O24.4H2O 0,20 g 

Vitamins solution

Component

Quantity Usedto 200 ml
cyanocobalamin 0,008 g 
thiamine HCl 0,008 g 
biotin 0,008 g 

 

Literature:

  • ANDERSEN, R.A. [ed.] 2005. Algal culturing techniques. Elsevier, Amsterdam, 578 pp. 
  • SCHLÖSSER, U.G., 1982. Sammlung von Algenkulturen. - Ber.Dt.bot.Ges. 95: 181-276.