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CCALA Culture Collection
of Autotrophic Organisms

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CCALA Prepares a New Licensing Framework under the PRAK Programme of the Czech Academy of Sciences

As part of the Programme for the Development of Applications and Commercialisation of the Czech Academy of Sciences — PRAK, the CCALA Culture Collection is preparing a new licensing and contractual framework for cooperation with companies and other application partners.

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Culture Collection of Autotrophic Organisms (CCALA)
Experimental Garden and Gene Pool Collections Třeboň
Institute of Botany of the CAS, v. v. i.
ccala@ibot.cas.cz

Jaworski’s Medium

Author: Schlösser, 1982

For freshwater algae.

To approximately 900 mL of dH₂O, add the appropriate volumes of all stock solutions. Bring the final volume to 1,000 mL with dH₂O.

Component	Stock Solution (g.l^-1 dH₂O)	Quantity Used to 1 litre
Ca(NO₃)₂.4H₂O	20,0	1 ml
KH₂PO₄	12,4	1 ml
MgSO₄.7H₂O	50,0	1 ml
NaHCO₃	15,9	1 ml
EDTA solution	see following recipe	1 ml
Trace metals solution	see following recipe	1 ml
NaNO₃	80,0	1 ml
Na₂HPO₄.12H₂O	36,0	1 ml
Vitamin solution	see following recipe	1 ml

EDTA-solution

Component	Quantity Used to 200 ml
FeNa-EDTA	0,45 g
Na₂EDTA	0,45 g

Trace metals solution

Component	Quantity Used to 200 ml
H₃BO₃	0,496 g
MnCl₂.4H₂O	0,278 g
(NH₄)₆Mo₇O₂₄.4H₂O	0,20 g

Vitamins solution

Component	Quantity Used to 200 ml
cyanocobalamin	0,008 g
thiamine HCl	0,008 g
biotin	0,008 g

Literature:

Schlösser, U. G. (1982). Sammlung von algenkulturen. Berichte der Deutschen Botanischen Gesellschaft, 95(1), 181-276.