Low-nutrient medium
Author: Schlösser, 1982 (= Basal medium)
To 905 mL of dH₂O, add the appropriate volumes of all stock solutions, soil extract, and micronutrient solution. Autoclave.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| KNO3 | 1 g | 20 ml |
| K2HPO4 | 0,1 g | 20 ml |
| MgSO4.7H2O | 0,1 g | 20 ml |
| Soil extract | see following recipe | 30 ml |
| Micronutrient solution | see following recipe | 5 ml |
Soil extract
Fill about one third of a flask with air-dried garden or leaf soil. Avoid soil with high humus or clay content, and do not use soil contaminated with fertilizers, herbicides, or fungicides. Add deionized water until the water level is about 5 cm above the soil. Sterilize the mixture for 1 hour, then repeat the sterilization after 24 hours. Let the soil settle and separate the clear extract by decanting, filtration, or centrifugation. Dispense the extract into small containers, autoclave for 20 minutes, and store in a refrigerator.'
Micronutrient solution
Autoclave the components separately in two solutions and combine them after cooling:
- Solution I: 881 ml of dH2O + stock solutions of salts
- Solution II: 100 ml of dH2O + FeSO4 + EDTA
| Component | Stock Solution (g to 100 ml dH2O) |
Quantity Used |
|---|---|---|
Solution I. |
||
| ZnSO4.7H2O | 0,1 g | 1 ml |
| MnSO4.4H2O | 0,1 g | 2 ml |
| H3BO3 | 0,2 g | 5 ml |
| Co(NO3)2.6H2O | 0,02 g | 5 ml |
| Na2MoO4.2H2O | 0,02 g | 5 ml |
| CuSO4.5H2O | 0,0005 g | 1 ml |
| Solution II. | ||
| FeSO4.7H2O | - | 0,7 g |
| EDTA | - | 0,8 g |
Literature:
- Schlösser, U. G. (1982). Sammlung von algenkulturen. Berichte der Deutschen Botanischen Gesellschaft, 95(1), 181-276.