Polytoma medium
Author: Schlösser, 1982
To 950 mL of dH₂O, add the appropriate volumes of all stock solutions and soil extract. Autoclave.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| Yeast extract | 10 g | 10 ml |
| Bacto-tryptone | 10 g | 10 ml |
| Soil extract | see following recipe | 30 ml |
Soil extract
Fill about one third of a flask with air-dried garden or leaf soil. Avoid soil with high humus or clay content, and do not use soil contaminated with fertilizers, herbicides, or fungicides. Add deionized water until the water level is about 5 cm above the soil. Sterilize the mixture for 1 hour, then repeat the sterilization after 24 hours. Let the soil settle and separate the clear extract by decanting, filtration, or centrifugation. Dispense the extract into small containers, autoclave for 20 minutes, and store in a refrigerator.
Literature:
- Schlösser, U. G. (1982). Sammlung von algenkulturen. Berichte der Deutschen Botanischen Gesellschaft, 95(1), 181-276.