Porphyridium medium
Authors: Brody & Emerson 1959, modif. Pekárková (pers. comm.)
Prepare Solution 1 and bring the final volume to 1,000 mL with dH₂O. Add 0.5 mL of Solution 2 and 0.5 mL of Solution 3. Autoclave.
Solution 1.
|
Component |
Quantity Used |
|---|---|
| KCl | 4,00 g |
| NaCl | 3,13 g |
| KNO3 | 1,24 g |
| MgSO4.7H2O | 2,50 g |
| K2HPO4 | 0,66 g |
| Ca(NO3)2.4H2O | 0,17 g |
| KI | 0,05 g |
| KBr | 0,05 g |
Solution 2.
|
Component |
Quantity Used |
|---|---|
| Fe-EDTA | 4,6 g |
Solution 3.
|
Component |
Quantity Used |
|---|---|
| H3BO3 | 3,09 mg |
| MnSO4.4H2O | 1,20 mg |
| CoSO4.7H2O | 1,40 mg |
| CuSO4.5H2O | 1,24 mg |
| ZnSO4.7H2O | 1,43 mg |
| (NH4)6Mo7O24.4H2O | 1,84 mg |
Literature:
- Brody, M., & Emerson, R. (1959). The effect of wavelength and intensity of light on the proportion of pigments in Porphyridium cruentum. American Journal of Botany, 46(6), 433-440.