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Culture Collection of Autotrophic Organisms (CCALA)
Experimental Garden and Gene Pool Collections Třeboň
Institute of Botany of the CAS, v. v. i.
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Porphyridium medium

Authors: Brody & Emerson 1959, modif. Pekárková (pers. comm.)

Mix Solution 1 with 0,5 ml of Solution 2 and 0,5 ml of Solution 3 and autoclave.
 

Solution 1.

Component

Quantity Usedto 1 litre

KCl 4,00 g 
NaCl 3,13 g 
KNO3 1,24 g
MgSO4.7H2O 2,50 g
K2HPO4 0,66 g
Ca(NO3)2.4H2O 0,17 g 
KI 0,05 g
KBr 0,05 g

Solution 2.

Component

Quantity Usedto 250 mililitres

Fe-EDTA 4,6 g 

Solution 3.

Component

Quantity Usedto 1 litre

H3BO3 3,09 mg
MnSO4.4H2O 1,20 mg
CoSO4.7H2O 1,40 mg
CuSO4.5H2O 1,24 mg
ZnSO4.7H2O 1,43 mg
(NH4)6Mo7O24.4H2O 1,84 mg

 

Literature:

  • BRODY,M. & EMERSON,R. (1959): The effect of wavelength and intensity of light on the proportion of pigments in Porphyridium cruentum. - Am.J.Bot. 46: 433-440.