WC medium
Authors: Guillard & Lorenzen, 1972
For planktonic algae and cyanobacteria.
To approximately 900 mL of dH₂O, add the appropriate volumes of all stock solutions and TRIS buffer, except for the vitamin solution. Bring the final volume to 1,000 mL with dH₂O and autoclave. After cooling, add the sterile-filtered vitamin solution aseptically.
| Component | Stock Solution (g.l-1 dH2O) |
Quantity Used to 1 litre |
|---|---|---|
| CaCl2.2H2O | 36,8 g | 1 ml |
| MgSO4.7H2O | 37,0 g | 1 ml |
| NaHCO3 | 12,6 g | 1 ml |
| K2HPO4.3H2O | 5,7 g | 1 ml |
| NaNO3 | 42,5 g | 1 ml |
| Na2SiO3.5H2O | 21, 2 g | 1 ml |
| trace metal solution | see bellow | 1 ml |
| vitamins solution | see bellow | 1 ml |
| TRIS | - | 0,115 g |
Trace metals solution
| Component | Quantity Used to 1 litre |
|---|---|
| Na2EDTA | 4,360 g |
| FeCl3.6H2O | 3,150 g |
| CuSO4.5H2O | 0,010 g |
| ZnSO4.7H2O | 0,022 g |
| CoCl2.6H2O | 0,010 g |
| MnCl2.4H2O | 0,180 g |
| Na2MoO4.2H2O | 0,006 g |
| H3BO3 | 1,000 g |
Vitamins solution
Sterile by filtration
| Component | Quantity Used to 1 litre |
|---|---|
| thiamin | 0,1 g |
| biotin | 0,0005 g |
| cyanokabalamin | 0,0005 g |
Literature:
- Guillard, R. R., & Lorenzen, C. J. (1972). Yellow-green algae with chlorophyllide c. Journal of Phycology, 8(1).