WC medium
Authors: Guillard & Lorenzen, 1972
For planktonic algae and cyanobacteria.
Combine 1 ml of stock solution and add TRIS buffer. Autoclave. Add sterille vitamin solution do cool medium.
| Component | Stock Solution | Quantity Used |
|---|---|---|
| CaCl2.2H2O | 36,8 g | 1 ml |
| MgSO4.7H2O | 37,0 g | 1 ml |
| NaHCO3 | 12,6 g | 1 ml |
| K2HPO4.3H2O | 5,7 g | 1 ml |
| NaNO3 | 42,5 g | 1 ml |
| Na2SiO3.5H2O | 21, 2 g | 1 ml |
| trace metal solution | see bellow | 1 ml |
| vitamins solution | see bellow | 1 ml |
| TRIS | - | 0,115 g |
Trace metals solution
| Component | Quantity Used |
|---|---|
| Na2EDTA | 4,360 g |
| FeCl3.6H2O | 3,150 g |
| CuSO4.5H2O | 0,010 g |
| ZnSO4.7H2O | 0,022 g |
| CoCl2.6H2O | 0,010 g |
| MnCl2.4H2O | 0,180 g |
| Na2MoO4.2H2O | 0,006 g |
| H3BO3 | 1,000 g |
Vitamins solution
Sterile by filtration
| Component | Quantity Used |
|---|---|
| thiamin | 0,1 g |
| biotin | 0,0005 g |
| cyanokabalamin | 0,0005 g |
Literature:
- GUILLARD, R.R.L., LORENZEN, C.J. (1972): Yellow-green algae with chlorophyllide C. Journal of Phycology 8: 10-14