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Culture Collection of Autotrophic Organisms (CCALA)
Experimental Garden and Gene Pool Collections Třeboň
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1/2 SŠ medium

Authors: Zachleder & Šetlík, 1982 

Combine 970 ml of dH2O and 10 ml of each stock solution. Autoclave. After cooling add 1,5 ml of 1N NaOH (=10g NaOH in 250 ml of dH2O.

For Euhalothece add 100g of Na2CO3 to 1 litre of medium.

 

Component

Stock Solutionto 1 litre of dH2O

Quantity Usedto 500 ml

Stock solution 1.

MgSO4.7H2O 98,8 g 10 ml
Trace metals elements (see following recipe) 100 ml 
 

Stock solution 2.

KNO3 202,0 g 10 ml
CaCl2.6H2O 1,1 g
Fe-EDTA 1,8 g
 

Stock solution 3.

KH2PO4 34,0 g  10 ml

Trace metals solution 

Component

Quantity Usedto 1 litre

H3BO3 3,086 g
MnSO4.4H2O 1,18 g
CoSO4.7H2O 1,404 g
CuSO4.5H2O 1,244 g
ZnSO4.7H2O 1,43 g
(MN4)6Mo7O24.4H2O 1,84 g

 

Literature: 

  • ZACHLEDER, V. & ŠETLÍK, I. 1982. Effect of Irradiance on the Course of RNA Synthesis in the Cell Cycle of Scenedesmus quadricauda. Biologia Plantarum 24(5): 341 - 353.