1/2 SŠ medium
Authors: Zachleder & Šetlík, 1982
Combine 970 ml of dH2O and 10 ml of each stock solution. Autoclave. After cooling add 1,5 ml of 1N NaOH (=10g NaOH in 250 ml of dH2O.
For Euhalothece add 100g of Na2CO3 to 1 litre of medium.
Component |
Stock Solution |
Quantity Used |
---|---|---|
Stock solution 1. |
||
MgSO4.7H2O | 98,8 g | 10 ml |
Trace metals elements (see following recipe) | 100 ml | |
Stock solution 2. |
||
KNO3 | 202,0 g | 10 ml |
CaCl2.6H2O | 1,1 g | |
Fe-EDTA | 1,8 g | |
Stock solution 3. |
||
KH2PO4 | 34,0 g | 10 ml |
Trace metals solution
Component |
Quantity Used |
---|---|
H3BO3 | 3,086 g |
MnSO4.4H2O | 1,18 g |
CoSO4.7H2O | 1,404 g |
CuSO4.5H2O | 1,244 g |
ZnSO4.7H2O | 1,43 g |
(MN4)6Mo7O24.4H2O | 1,84 g |
Literature:
- ZACHLEDER, V. & ŠETLÍK, I. 1982. Effect of Irradiance on the Course of RNA Synthesis in the Cell Cycle of Scenedesmus quadricauda. Biologia Plantarum 24(5): 341 - 353.