Jump to

Search by filter

In any taxon

From any country

From any habitat

By any author

From any year

In any medium

Find corresponding strains

Taxonomy

CCALA Culture Collection
of Autotrophic Organisms

News

CCALA Prepares a New Licensing Framework under the PRAK Programme of the Czech Academy of Sciences

As part of the Programme for the Development of Applications and Commercialisation of the Czech Academy of Sciences — PRAK, the CCALA Culture Collection is preparing a new licensing and contractual framework for cooperation with companies and other application partners.

.

Ordering rules

We accept only signed CCALA order forms send by post or their scans send by the e-mail. We accept your original institutional orders too. By submitting the online form, you explicitly agree with our terms and conditions.

Read Terms & Conditions

Contact

Culture Collection of Autotrophic Organisms (CCALA)
Experimental Garden and Gene Pool Collections Třeboň
Institute of Botany of the CAS, v. v. i.
ccala@ibot.cas.cz

1/2 SŠ medium

Authors: Zachleder & Šetlík, 1982

To 970 mL of dH₂O, add the appropriate volumes of all stock solutions and autoclave. After cooling, add 1.5 mL of 1 N NaOH aseptically

Component	Stock Solution (g.l^-1 dH₂O)	Quantity Used to 500 ml
Stock solution 1.
MgSO₄.7H₂O	98,8 g	10 ml
Trace metals elements (see following recipe)	100 ml

Stock solution 2.
KNO₃	202,0 g	10 ml
CaCl₂.6H₂O	1,1 g
Fe-EDTA	1,8 g

Stock solution 3.
KH₂PO₄	34,0 g	10 ml

Trace metals solution

Component	Quantity Used to 1 litre
H₃BO₃	3,086 g
MnSO₄.4H₂O	1,18 g
CoSO₄.7H₂O	1,404 g
CuSO₄.5H₂O	1,244 g
ZnSO₄.7H₂O	1,43 g
(MN₄)₆Mo₇O₂₄.4H₂O	1,84 g

Medium modification:

Euhalothece medium modification: For Euhalothece (CCALA 947), add 100 g of Na₂CO₃ to 1 litre of ½ SŠ medium.

Literature:

Zachleder, V., & Šetlík, I. (1982). Effect of irradiance on the course of RNA synthesis in the cell cycle of Scenedesmus quadricauda. Biologia Plantarum, 24(5), 341-353.