BBM medium
Authors: Bold 1949, Bischoff and Bold 1963
Useful for many algae, especially chlorococcalea algae, volvocalean algae (e.g.Chlamydomonas), filamentous green alga Klebsormidim flaccidum, xanthophycean alga Heterococcus endolithicus etc. (Andersen, 2005).
Into 900 ml of dH2O add 10 ml of first six stock solutions. Add 1 ml of rest solutions. Autoclave. The final pH should be 6,6
Component |
Stock Solution |
Quantity Used |
---|---|---|
NaNO3 | 25,00 | 10 ml |
CaCl2.2H2O | 2,50 | 10 ml |
MgSO4.7H2O | 7,50 | 10 ml |
K2HPO4 | 7,50 | 10 ml |
KH2PO4 | 17,50 | 10 ml |
NaCl | 2,50 | 10 ml |
EDTA solution | see following recipe | 1 ml |
Acidifed iron solution | see following recipe | 1 ml |
H3BO3 | 11,42 g | 1 ml |
Trace metals solution | see following recipe | 1 ml |
EDTA solution
Component |
Quantity Used |
---|---|
EDTA | 50,00 g |
KOH | 31,00 g |
Acidified Iron Solution
Component |
Quantity Used |
---|---|
FeSO4.7H2O | 0,498 g |
H2SO4 (96%) | 0,1 ml |
Trace metals solution
Component |
Quantity Used |
---|---|
ZnSO4.7H2O | 8,82 g |
MnCl2.4H2O | 1,44 g |
MoO3 | 0,71 g |
CuSO4.5H2O | 1,57 g |
Co(NO3)2.6H2O | 0,49 g |
Literature:
- ANDERSEN, R.A. [ed.] 2005. Algal culturing techniques. Elsevier, Amsterdam, 578 pp.
- BISCHOFF, H.W., and BOLD, H.C. 1963.Phycological Studies IV. Some Soil Algae From Enchanted Rock and Related Algal Specie. University of Texas, Austin, 6318: 1 - 95.
- BOLD, H.C. 1949. The morphology of Chlamydomonas chlamydogama sp. nov.. Bull. Torrey Bot. Club. 76: 101 - 108.