BBM medium
Authors: Bold 1949; Bischoff and Bold 1963
To 900 mL of dH₂O, add the appropriate volumes of all stock solutions. Bring the final volume to 1,000 mL with dH₂O and autoclave. The final pH should be 6.6.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| NaNO3 | 25,00 | 10 ml |
| CaCl2.2H2O | 2,50 | 10 ml |
| MgSO4.7H2O | 7,50 | 10 ml |
| K2HPO4 | 7,50 | 10 ml |
| KH2PO4 | 17,50 | 10 ml |
| NaCl | 2,50 | 10 ml |
| EDTA solution | see following recipe | 1 ml |
| Acidifed iron solution | see following recipe | 1 ml |
| H3BO3 | 11,42 g | 1 ml |
| Trace metals solution | see following recipe | 1 ml |
EDTA solution
| Component |
Quantity Used |
|---|---|
| EDTA | 50,00 g |
| KOH | 31,00 g |
Acidified Iron Solution
| Component |
Quantity Used |
|---|---|
| FeSO4.7H2O | 0,498 g |
| H2SO4 (96%) | 0,1 ml |
Trace metals solution
| Component |
Quantity Used |
|---|---|
| ZnSO4.7H2O | 8,82 g |
| MnCl2.4H2O | 1,44 g |
| MoO3 | 0,71 g |
| CuSO4.5H2O | 1,57 g |
| Co(NO3)2.6H2O | 0,49 g |
Medium modification:
- 3N-BBM modification: For this modification, add 30 ml of stock solution No. 1 when preparing the medium.
Literature:
- Bischoff, H. W. (1963). Phycological studies IV. Some soil algae from Enchanted Rock and related algal species. University of Texas Publication, 6318, 1.
- Bold, H. C. (1949). The morphology of Chlamydomonas chlamydogama, sp. nov. Bulletin of the Torrey Botanical Club, 101-108.