BG11 medium
Authors: Allen 1968, Allen and Stainer 1968, Rippka et al. 1979
For culture of freshwater, soil, thermal and marine cyanobacteria (Andersen, 2005).
Into 900 ml of dH2O add 1 ml of the Fe-citrate solution, and then add remaining components. Autoclave. Final pH should be 7,4 after cooling.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| Fe-citrate solution | see following recipe | 1 ml |
| NaNO3 | - | 1,5 g |
| K2HPO4.3H3O | 40 | 1 ml |
| MgSO4.7H2O | 75 | 1 ml |
| CaCl2.2H2O | 35 | 1 ml |
| Na2CO3 | 20 | 1 ml |
| Na-EDTA | 1,0 | 1 ml |
| Trace metals solution | see following recipe | 1 ml |
Fe-citrate solution
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| Citric acid | 6 | 1 ml |
| Ferric ammonium citrate | 6 | 1 ml |
Trace metals solution
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| H3BO3 | - | 2,860 g |
| MnCl2.4H2O | - | 1,810 g |
| ZnSO4.7H2O | - | 0,220 g |
| CuSO4.5H2O | 79,0 | 1 ml |
| Na2MoO4.2H2O | - | 0,391 g |
| Co(NO3)2.6H2O | 49,4 | 1 ml |
Literature:
- ALLEN, M.M. 1968. Simple conditions for growth of unicellular blue-green algae. J. Gen. Microbiol. 51: 199 - 202.
- ALLEN, M.M., and STAINER, R.Y. 1968. Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch. Mikrobiol. 32: 270 - 277.
- ANDERSEN, R.A. [ed.] 2005. Algal culturing techniques. Elsevier, Amsterdam, 578 pp.
- RIPKA, R., DERUELLES, J., WATERBURY, J.B., HERDMAN, M., and STAINER, R.Y. 1979. Generic assignment, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111: 1 - 61.