BG11 medium
Authors: Allen, 1959, 1968; Rippka et al., 1979
To 800 mL of dH₂O, add the appropriate volumes of all stock solutions and the trace metals solution. Autoclave. The final pH should be 7.4 after cooling.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| NaNO₃ | 15,0 | 100 ml |
| K₂HPO₄ | 4,0 | 10 ml |
| MgSO₄·7H₂O | 7,5 | 10 ml |
| CaCl₂·2H₂O | 3,6 | 10 ml |
| citric acid | 0,6 | 10 ml |
| ferric ammonium citrate | 0,6 | 10 ml |
| Na₂EDTA | 0,1 | 10 ml |
| Na₂CO₃ | 2,0 | 10 ml |
| Trace metals solution | see following recipe | 1 ml |
Trace metals solution
| Component |
Quantity Used |
|---|---|
| H3BO3 | 2,860 g |
| MnCl2.4H2O | 1,810 g |
| ZnSO4.7H2O | 0,220 g |
| Na₂MoO₄·2H₂O | 0,391 g |
| CuSO₄·5H₂O | 0,080 g |
| Co(NO₃)₂·6H₂O | 0,050 g |
Medium modifications:
- Oxynema medium modification: For Oxynema (CCALA 960), add 30 g of NaCl to 1 litre of BG11 medium.
- BG11-0/BG11-N modification: For nitrogen-fixing cyanobacteria, prepare standard BG11 medium without Stock 1.
Literature:
- Allen, M. B. (1959). Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Archiv für Mikrobiologie, 32(3), 270-277.
- Allen, M. M. (1968). Simple conditions for growth of unicellular blue‐green algae on plates 1, 2. Journal of phycology, 4(1), 1-4.
- Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M., & Stanier, R. Y. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology, 111(1), 1-61.