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Culture Collection of Autotrophic Organisms (CCALA)
Experimental Garden and Gene Pool Collections Třeboň
Institute of Botany of the CAS, v. v. i.
ccala@ibot.cas.cz

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BG11 medium

Authors: Allen 1968, Allen and Stainer 1968, Rippka et al. 1979

For culture of freshwater, soil, thermal and marine cyanobacteria (Andersen, 2005).

Into 900 ml of dH2O add 1 ml of the Fe-citrate solution, and then add remaining components. Autoclave. Final pH should be 7,4 after cooling.
 
Component

Stock Solutiong.l-1 dH2O

Quantity Usedto 1 litre

Fe-citrate solution see following recipe 1 ml
NaNO3 - 1,5 g
K2HPO4.3H3O 40 1 ml
MgSO4.7H2O 75 1 ml
CaCl2.2H2O 35 1 ml
Na2CO3 20 1 ml
Na-EDTA 1,0 1 ml
Trace metals solution see following recipe 1 ml

Fe-citrate solution  

Component

Stock Solutiong.l-1 dH2O

Quantity Usedto 1 litre

Citric acid 1 ml
Ferric ammonium citrate 6 1 ml

Trace metals solution 

Component

Stock Solutiong.l-1 dH2O

Quantity Usedto 1 litre

H3BO3 - 2,860 g
MnCl2.4H2O - 1,810 g
ZnSO4.7H2O - 0,220 g
CuSO4.5H2O 79,0 1 ml
Na2MoO4.2H2O - 0,391 g
Co(NO3)2.6H2O 49,4 1 ml

 

Literature:

  • ALLEN, M.M. 1968. Simple conditions for growth of unicellular blue-green algae. J. Gen. Microbiol. 51: 199 - 202.
  • ALLEN, M.M., and STAINER, R.Y. 1968. Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch. Mikrobiol. 32: 270 - 277. 
  • ANDERSEN, R.A. [ed.] 2005Algal culturing techniques. Elsevier, Amsterdam, 578 pp. 
  • RIPKA, R., DERUELLES, J., WATERBURY, J.B., HERDMAN, M., and STAINER, R.Y. 1979. Generic assignment, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111: 1 - 61.