Brackish medium
Author: Schlösser, 1982
Combine dH₂O and artificial seawater, then add the appropriate volumes of all stock solutions, soil extract, and trace metal solution. Bring the final volume to 1,000 mL with dH₂O.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| KNO3 | 1,00 | 20 ml |
| K2HPO4 | 0,1 g | 20 ml |
| MgSO4.7H2O | 0,1 g | 20 ml |
| Soil extract | see following recipe | 30 ml |
| Trace metal solution | see following recipe | 5 ml |
| dH2O | - | 450 ml |
| Artificial seawater | see following recipe | 450 ml |
Soil extract
Fill about one third of a flask with air-dried garden or leaf soil. Avoid soil with high humus or clay content, and do not use soil contaminated with fertilizers, herbicides, or fungicides. Add deionized water until the water level is about 5 cm above the soil. Sterilize the mixture for 1 hour, then repeat the sterilization after 24 hours. Let the soil settle and separate the clear extract by decanting, filtration, or centrifugation. Dispense the extract into small containers, autoclave for 20 minutes, and store in a refrigerator.
Trace metals solution
| Component |
Quantity Used |
|---|---|
| ZnSO4.7H2O | 8,82 g |
| MnCl2.4H2O | 1,44 g |
| MoO3 | 0,71 g |
| CuSO4.5H2O | 1,57 g |
| Co(NO3)2.6H2O | 0,49 g |
Artificial seawater
| Component |
Quantity Used |
|---|---|
| NaCl | 30,0 g |
| MgSO4.7H2O | 10,0 g |
| KCl | 1,5 g |
| CaSO4.2H2O | 2,0 g |
Literature:
- Schlösser, U. G. (1982). Sammlung von algenkulturen. Berichte der Deutschen Botanischen Gesellschaft, 95(1), 181-276.