Brackish water
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| KNO3 | 1,00 | 20 ml |
| K2HPO4 | 0,1 g | 20 ml |
| MgSO4.7H2O | 0,1 g | 20 ml |
| Soil extract | see following recipe | 30 ml |
| Trace metal solution | see following recipe | 5 ml |
| dH2O | - | 450 ml |
| Artificial seawater | see following recipe | 450 ml |
Soil extract
1/3 of 6 litre flask fill with garden or leaf soil (not too great humus or clay content), and deionized water until it stands 5cm above the soil. Sterilize by heating in a steamer one hour, twice in a 24h interval. Separate the decanted extract by centrifugation. Fill into small containers, autoclave 20 minutes/1 atmosphere; store in refrigerator.
Trace metals solution
| Component |
Quantity Used |
|---|---|
| ZnSO4.7H2O | 8,82 g |
| MnCl2.4H2O | 1,44 g |
| MoO3 | 0,71 g |
| CuSO4.5H2O | 1,57 g |
| Co(NO3)2.6H2O | 0,49 g |
Artificial seawater
| Component |
Quantity Used |
|---|---|
| NaCl | 30,0 g |
| MgSO4.7H2O | 10,0 g |
| KCl | 1,5 g |
| CaSO4.2H2O | 2,0 g |