Dunaliella medium
Author: Schlösser 1982
The cultivation of halophilic algae.
Mix 20 ml of the stock macroelements solution with 910 ml of artificial seawater and 30 ml of seawater soil extract.
Component |
Stock Solution |
Quantity Used |
---|---|---|
KNO3 | 10,00 g | 10 ml |
K2HPO4 | 1,00 g | 10 ml |
MgSO4.7H2O | 1,00 g | 10 ml |
soil extract | see following recipe | 30 ml |
artificial seawater | see following recipe | 930 ml |
Soil extract
1/3 of 6 litre flask fill with garden or leaf soil (not too great humus or clay content), and deionized water until it stands 5cm above the soil. Sterilize by heating in a steamer one hour, twice in a 24h interval. Separate the decanted extract by centrifugation. Fill into small containers, autoclave 20 minutes/1 atmosphere; store in refrigerator.
Artificial seawater
Component |
Quantity Used |
---|---|
NaCl | 30,0 g |
MgSO4.7H2O | 31,0 g |
KCl | 1,5 g |
CaSO4 | 2,0 g |
Literature:
- Andersen, R.A. [ed.] 2005. Algal culturing techniques. Elsevier, Amsterdam, 578 pp.
- Schlösser, U.G., 1982. Sammlung von Algenkulturen. - Ber.Dt.bot.Ges. 95: 181-276.