News

Our collection is now also on the X platform - feel free to follow us https://x.com/CCALAcollection

View all news

Ordering rules

We accept only signed CCALA order forms send by post or their scans send by the e-mail. We accept your original institutional orders too. By submitting the online form, you explicitly agree with our terms and conditions.

Read Terms & Conditions

Contact

Culture Collection of Autotrophic Organisms (CCALA)
Experimental Garden and Gene Pool Collections Třeboň
Institute of Botany of the CAS, v. v. i.
ccala@ibot.cas.cz

Go to contacts

Dunaliella medium

Author: Schlösser 1982

The cultivation of halophilic algae.

Mix 20 ml of the stock macroelements solution with 910 ml of artificial seawater and 30 ml of seawater soil extract.

 

Component

Stock Solutiong.l-1 dH2O

Quantity Usedto 1 litre

KNO3 10,00 g 10 ml
K2HPO4 1,00 g  10 ml
MgSO4.7H2O 1,00 g 10 ml
soil extract see following recipe 30 ml
artificial seawater see following recipe 930 ml

Soil extract

1/3 of 6 litre flask fill with garden or leaf soil (not too great humus or clay content), and deionized water until it stands 5cm above the soil. Sterilize by heating in a steamer one hour, twice in a 24h interval. Separate the decanted extract by centrifugation. Fill into small containers, autoclave 20 minutes/1 atmosphere; store in refrigerator.
 

Artificial seawater 

Component

Quantity Usedg.l-1 dH2O

NaCl 30,0 g
MgSO4.7H2O 31,0 g 
KCl 1,5 g
CaSO4 2,0 g

 

Literature:

  • Andersen, R.A. [ed.] 2005. Algal culturing techniques. Elsevier, Amsterdam, 578 pp. 
  • Schlösser, U.G., 1982. Sammlung von Algenkulturen. - Ber.Dt.bot.Ges. 95: 181-276.