CCALA Culture Collection
of Autotrophic Organisms

Dunaliella medium

Author: Schlösser, 1982

To 900 mL of dH₂O, add the appropriate volumes of all stock solutions and soil extract. Bring the final volume to 1,000 mL with dH₂O. Autoclave.

Soil extract

Fill about one third of a flask with air-dried garden or leaf soil. Avoid soil with high humus or clay content, and do not use soil contaminated with fertilizers, herbicides, or fungicides. Add deionized water until the water level is about 5 cm above the soil. Sterilize the mixture for 1 hour, then repeat the sterilization after 24 hours. Let the soil settle and separate the clear extract by decanting, filtration, or centrifugation. Dispense the extract into small containers, autoclave for 20 minutes, and store in a refrigerator.

Double-strength artificial seawater

Literature:

• Schlösser, U. G. (1982). Sammlung von algenkulturen. Berichte der Deutschen Botanischen Gesellschaft, 95(1), 181-276.