Galdieria medium - modified
Authors: Gross, W. & Schnarrenberger, 1995
Dissolve chemicals in 500 ml of distilled water. Add 2,07 ml of Fe-EDTA solution and 2,00 ml trace metal solution. Make up to 1000 ml. Autoclave.
Adjust medium to final pH of 1.8 or as desired with 0.5 M H2SO4
|
Component |
Quantity Used |
|---|---|
| (NH4)2SO4 | 1,5 g |
| MgSO4.7H2O | 0,3 g |
| KH2PO4 | 0,3 g |
| CaCl2.2H2O | 0,02 g |
| Fe-EDTA (see following recipe) | 2,07 ml |
| Trace metals solution (see following recipe) | 2,00 ml |
Fe-EDTA solution
To 50 ml od dH2O add following component and fill in with dH2O do 100 ml.
| Component |
Quantity Used |
|---|---|
| KOH (1 N solution) | 5,4 ml |
| FeSO4.7H2O | 0,5 g |
| EDTA (no Na-EDTA!) | 0,52 g |
Trace metals solution
| Component |
Quantity Used |
|---|---|
| ZnSO4.7H2O | 220 mg |
| H3BO3 | 2860 mg |
| CoCl2.6H2O | 40 mg |
| CuSO4.5H2O | 79 mg |
| (NH4)2Mo7O27.6H2O | 130 mg |
| NaVO3 | 40 mg |
| MnCl2.4H2O | 1790 mg |
Literautre:
- Gross, W. & Schnarrenberger, C. 1995. Heterotrophic Growth of Two Strains of the Acido-Thermophilic Red Alga Galdieria sulphuraria. Plant Cell. Physiol. 36(4): 633 - 638.