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Galdieria medium - modified

Authors: Gross, W. & Schnarrenberger, 1995

Dissolve chemicals in 500 ml of distilled water. Add 2,07 ml of Fe-EDTA solution and 2,00 ml trace metal solution. Make up to 1000 ml. Autoclave.

Adjust medium to final pH of 1.8 or as desired with 0.5 M H2SO4 

Component

Quantity Usedto 1 litre
(NH4)2SO4 1,5 g
MgSO4.7H2O 0,3 g
KH2PO4 0,3 g 
CaCl2.2H2O 0,02 g
Fe-EDTA (see following recipe) 2,07 ml
Trace metals solution (see following recipe) 2,00 ml 

Fe-EDTA solution 

To 50 ml od dH2O add following component and fill in with dH2O do 100 ml.
 

Component

Quantity Used
KOH (1 N solution) 5,4 ml
FeSO4.7H2O 0,5 g
EDTA (no Na-EDTA!) 0,52 g 

Trace metals solution 

Component

Quantity Usedto 1 litre
ZnSO4.7H2O 220 mg
H3BO3 2860 mg
CoCl2.6H2O 40 mg
CuSO4.5H2O 79 mg
(NH4)2Mo7O27.6H2O 130 mg
NaVO3 40 mg
MnCl2.4H2O 1790 mg

Literautre: 

  • Gross, W. & Schnarrenberger, C. 1995. Heterotrophic Growth of Two Strains of the Acido-Thermophilic Red Alga Galdieria sulphuraria. Plant Cell. Physiol. 36(4): 633 - 638.