Gorham’s solution
Authors: Koeman & van den Hoek, 1981
Dissolve the appropriate amounts of all components in dH₂O add the soil extract. Bring the final volume to 1,000 mL with dH₂O.
|
Component |
Quantity Used |
|---|---|
| NaNO3 | 160 mg |
| K2HPO4 | 17,5 mg |
| TRIS buffer | 200 mg |
| MgSO4.7H2O | 153,6 mg |
| CuSO4.5H2O | 0,0156 mg |
| CaCl2 | 35 mg |
| Na2SiO3 | 11,63 mg |
| Na2CO3 | 20 mg |
| Fe-citrate | 3 mg |
| Citric acid | 3 mg |
| Na-EDTA (Chelaton III) | 600 mg |
| Na2MoO4.2H2O | 0,00705 mg |
| ZnSO4.7H2O | 0,0392 mg |
| CoCl2 | 0,0183 mg |
| MnCl2.4H2O | 0,283 mg |
Soil extract
1/3 of 6 litre flask fill with garden or leaf soil (not too great humus or clay content), and deionized water until it stands 5cm above the soil. Sterilize by heating in a steamer one hour, twice in a 24h interval. Separate the decanted extract by centrifugation. Fill into small containers, autoclave 20 minutes/1 atmosphere; store in refrigerator.
Literature:
- Koeman, R. P. T., & van den Hoek, C. (1981). The taxonomy of Ulva (Chlorophyceae) in the Netherlands. British Phycological Journal, 16(1), 9-53.