HS medium for Chlamydomonas
Author: Sueoka 1960
Into 500 ml of dH2O add quantity of stock solutions (see belowes) fill in 990 mililitres with dH2O. Autoclave. After cooling add 10 ml of stock solution no. 5.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
Stock stolution 1. |
||
| NH4Cl | 50,00 | 10 ml |
| CaCl2.2H2O | 2,00 | |
Stock solution 2. |
||
| MgSO4.7H2O | 20,00 | 10 ml |
| FeNa-EDTA | 1,84 | |
Stock solution 3. |
||
| Na2MoO4.2H2O | 0,5 g to 100 ml dH2O | 0,5 ml |
Stock solution 4. |
||
| Trace metals solution | see following recipe | 1 ml |
Stock solution 5. |
||
| Add after autoclaving! | ||
| KH2PO4 | 72,00 | 10 ml |
| K2HPO4 | 134,00 | |
Trace metals solution
| Component |
Quantity Used |
|---|---|
| H3BO3 | 3,086 g |
| MnSO4.4H2O | 1,180 g |
| CoSO4.7H2O | 1,404 g |
| CuSO4.5H2O | 1,244 g |
| ZnSO4.7H2O | 1,430 g |
| (NH4)6Mo7O24.4H2O | 1,840 g |
Literature:
- Sueoka, N. 1960. MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI. PNAS 46(1): 83-91.