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Culture Collection of Autotrophic Organisms (CCALA)
Experimental Garden and Gene Pool Collections Třeboň
Institute of Botany of the CAS, v. v. i.
ccala@ibot.cas.cz
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Medium for non-seed plants
Authors: Baslerová & Dvořáková 1962
Liverworts, ferns and duckweeds are maintained in 250 ml Erlenmayer's flasks on agar medium in the air conditioned room at 12-15°C. They are irradiated similarly as other strains (see above).
Into 900 ml of dH2O add quantity of stock solutions (see belowe) fill in 1 litre with dH2O. Autoclave.
| Component |
Stock Solutiong.l-1 dH2O
|
Quantity Usedto 1 litre |
| |
Mosses (M)
|
Liverworts & ferns (JKL)
|
| NH4NO3 |
80,00 |
1.5 ml |
2.5 ml |
| KH2PO4 |
136,00 |
0.5 ml |
0.75 ml |
| MgSO4.7H2O |
120,00 |
0.5 ml |
0.4 ml |
| CaCl2.2H2O |
110,00 |
0.2 ml |
1,0 ml |
| FeCl3.6H2O |
16.20 |
0.2 ml |
0.16 ml |
Literature:
- Baslerová, M. & Dvořáková, J. 1962. Algarum, Hepaticarum Muscurumque in cultiris collectio. NCSAV, Praha, 59 pp.