Medium for non-seed plants
Authors: Baslerová & Dvořáková, 1962
Mosses, liverworts, and ferns are maintained in 250 mL Erlenmeyer flasks on agar medium in an air-conditioned room at 12–15 °C.
To 900 mL of dH₂O, add the appropriate volumes of all stock solutions for the selected medium variant (M or JKL) as indicated in the table. Bring the final volume to 1,000 mL with dH₂O and autoclave.
| Component |
Stock Solution (g.l-1 dH2O) |
Quantity Used to 1 litre |
|
|---|---|---|---|
| Mosses (M) | Liverworts & ferns (JKL) | ||
| NH4NO3 | 80,00 | 1,5 ml | 2,5 ml |
| KH2PO4 | 136,00 | 0,5 ml | 0,75 ml |
| MgSO4.7H2O | 120,00 | 0,5 ml | 0,4 ml |
| CaCl2.2H2O | 110,00 | 0,2 ml | 1,0 ml |
| FeCl3.6H2O | 16,20 | 0,2 ml | 0,16 ml |
Literature:
- Baslerová, M., & Dvořáková, J. (1962). Algarum, Hepaticarum, Muscorumque in culturis collectio. Nakl. Československé akademie věd.