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We accept only signed CCALA order forms send by post or their scans send by the e-mail. We accept your original institutional orders too. By submitting the online form, you explicitly agree with our terms and conditions.

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Contact

Culture Collection of Autotrophic Organisms (CCALA)
Experimental Garden and Gene Pool Collections Třeboň
Institute of Botany of the CAS, v. v. i.
ccala@ibot.cas.cz

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S medium

Dissolve the chemicals in 500 ml of distilled water, add 1 ml of trace metals solution and make up to 1000 ml. Autoclave.

 

Component

Quantity Usedto 1 litre

KCl 1,0 g
NaHCO3 16,8 g
K2HPO4 0,5 g
NaNO3 2,5 g
K2SO4 1,0 g
NaCl 30,0 g
MgSO4.7H2O 0,18 g
FeSO4.7H2O 0,018 g
EDTA 0,08 g
Trace metal solution 1 ml

Trace metals solution 

Component

Stock solutiong.l-1 dH2O

Quantity Usedto 1 litre

H3BO3 - 2,860 g
MnCl2.4H2O - 1,810 g
ZnSO4.7H2O - 0,220 g
CuSO4.5H2O 79,0 1 ml
Na2MoO4.2H2O - 0,391 g
Co(NO3)2.6H2O 49,4 1 ml