S medium
Authors: Gerasimenko et al., 1996; Samylina et al., 2021
Dissolve the chemicals in 500 mL of distilled water. Add 1,00 ml of trace metal solution and make up to 1000 ml with distilled water. Autoclave.
| Component |
Quantity Used |
|---|---|
| KCl | 1,0 g |
| NaHCO3 | 16,8 g |
| K2HPO4 | 0,5 g |
| NaNO3 | 2,5 g |
| K2SO4 | 1,0 g |
| NaCl | 30,0 g |
| MgSO4.7H2O | 0,18 g |
| FeSO4.7H2O | 0,018 g |
| EDTA | 0,08 g |
| Trace metal solution | 1 ml |
Trace metals solution
| Component |
Stock solution (g.l-1 dH2O) |
Quantity Used |
|---|---|---|
| H3BO3 | - | 2,860 g |
| MnCl2.4H2O | - | 1,810 g |
| ZnSO4.7H2O | - | 0,220 g |
| CuSO4.5H2O | 79,0 | 1 ml |
| Na2MoO4.2H2O | - | 0,391 g |
| Co(NO3)2.6H2O | 49,4 | 1 ml |
Literature:
- Gerasimenko, L. M., Dubinin, A. V., & Zavarzin, G. A. (1996). Alkaliphilic cyanobacteria from soda lakes of Tuva and their ecophysiology. Microbiology (Mikrobiologiya), 65(6), 736-740.
- Samylina, O. S., Sinetova, M. A., Kupriyanova, E. V., Starikov, A. Y., Sukhacheva, M. V., Dziuba, M. V., & Tourova, T. P. (2021). Ecology and biogeography of the ‘marine Geitlerinema’cluster and a description of Sodalinema orleanskyi sp. nov., Sodalinema gerasimenkoae sp. nov., Sodalinema stali sp. nov. and Baaleninema simplex gen. et sp. nov.(Oscillatoriales, Cyanobacteria). FEMS Microbiology Ecology, 97(8), fiab104.