Spirulina medium
Authors: Aiba and Ogawa 1977, Schlösser 1994
For culturing Spirulina.
Prepare Solutions I and II. Autoclave separately and cool; asepticilly combine the two solutions. Aseptically add 1 ml of cyanocobalamin (B12) solution.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| Solution I. (500 ml) | ||
| NaHCO3 | - | 13,61 g |
| Na2CO3 | - | 4,03 g |
| K2HPO4 | - | 0,50 g |
| Solution II. (500 ml) | ||
| NaNO3 | - | 2,5 g |
| K2SO4 | - | 1,0 g |
| NaCl | - | 1,0 g |
| MgSO4.7H2O | - | 0,2 g |
| CaCl2.2H2O | - | 0,04 g |
| FeSO4.7H2O | - | 0,01 g |
| Na2EDTA.2H2O | - | 0,08 g |
| Trace metals solution | see following recipe | 5 ml |
| Vitamins solution | see following recipe | 1 ml |
Trace metals solution
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| Na2EDTA.2H2O | - | 0,5 g |
| FeSO4.7H2O | - | 0,7 g |
| ZnSO4.7H2O | 1,0 | 1 ml |
| MnSO4.7H2O | 2,0 | 1 ml |
| H3BO3 | 10,0 | 1 ml |
| Co(NO3)2.6H2O | 1,0 | 1 ml |
| Na2MoO4.2H2O | 1,0 | 1 ml |
| CuSO4.5H2O | 0,005 | 1 ml |
Vitamin Solution
Dissolve the cyanocobalamin in 1 liter dH2O. Filter-sterilize and store frozen.
| Component |
Quantity Used |
|---|---|
| Cyanocobalamin (vit. B12) | 5 mg |
Literauture:
- AIBA, S., and OGAWA, T. 1977. Assessment of growth yield of a blue-green alga:Spirulina platensis in axenic and continuous cultur. J. Gen. Microbiol. 102: 179 - 182.
- ANDERSEN, R.A. [ed.] 2005. Algal culturing techniques. Elsevier, Amsterdam, 578 pp.
- SCHLÖSSER, U.G. 1994. SAG-Sammlung von Algenkulturen at the University of Göttingen Catalogue of Strains 1994. Bot. Acta 107: 111 - 186.