Spirulina medium
Authors: Aiba & Ogawa, 1977; Schlösser, 1994
Prepare Solutions I and II separately and bring each solution to a final volume of 500 mL with dH₂O. Autoclave separately and allow to cool. Combine the solutions aseptically, then add 1 mL of cyanocobalamin (vitamin B₁₂) solution aseptically.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| Solution I. (500 ml) | ||
| NaHCO3 | - | 13,61 g |
| Na2CO3 | - | 4,03 g |
| K2HPO4 | - | 0,50 g |
| Solution II. (500 ml) | ||
| NaNO3 | - | 2,5 g |
| K2SO4 | - | 1,0 g |
| NaCl | - | 1,0 g |
| MgSO4.7H2O | - | 0,2 g |
| CaCl2.2H2O | - | 0,04 g |
| FeSO4.7H2O | - | 0,01 g |
| Na2EDTA.2H2O | - | 0,08 g |
| Trace metals solution | see following recipe | 5 ml |
| Vitamins solution | see following recipe | 1 ml |
Trace metals solution
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| Na2EDTA.2H2O | - | 0,5 g |
| FeSO4.7H2O | - | 0,7 g |
| ZnSO4.7H2O | 1,0 | 1 ml |
| MnSO4.7H2O | 2,0 | 1 ml |
| H3BO3 | 10,0 | 1 ml |
| Co(NO3)2.6H2O | 1,0 | 1 ml |
| Na2MoO4.2H2O | 1,0 | 1 ml |
| CuSO4.5H2O | 0,005 | 1 ml |
Vitamin Solution
Dissolve the cyanocobalamin in 1 liter dH2O. Filter-sterilize and store frozen.
| Component |
Quantity Used |
|---|---|
| Cyanocobalamin (vit. B12) | 5 mg |
Literauture:
- Aiba, S., & Ogawa, T. (1977). Assessment of growth yield of a blue—green alga, Spirulina platensis, in axenic and continuous culture. Microbiology, 102(1), 179-182.
- Schlösser, U. G. (1994). SAG‐sammlung von algenkulturen at the University of Göttingen catalogue of strains 1994. Botanica acta, 107(3), 113-186.