Z medium
Authors: Staub, 1961
To 900 mL of dH₂O, add the appropriate volumes of all stock solutions. Bring the final volume to 1,000 ml with dH₂O. Autoclave. The final pH should be 6.6.
| Component |
Stock Solution |
Quantity Used |
|---|---|---|
| NaNO3 | 46,7 | 10 ml |
| Ca(NO3)2.4H2O | 5,9 | 10 ml |
| K2HPO4 | 3,1 | 10 ml |
| MgSO4.7H2O | 2,5 | 10 ml |
| Na2CO3 | 2,1 | 10 ml |
| Fe-EDTA solution | see following recipe | 0,2 ml |
| Trace metals sollution | see following recipe | 0,08 ml |
Fe-EDTA solution
| Component |
Quantity Used |
|---|---|
| HCl (35%) | 2,2 ml |
| dH2O | 250 ml |
| FeCl3.6H2O | 4,5 g |
| Na2EDTA | 4,65 g |
Trace metals solution
| Component |
Quantity Used |
|---|---|
| H3BO3 | 0,31 g |
| MnSO4.4H2O | 0,223 g |
| Na2WO4.2H2O | 0,003 g |
| (NH4)6Mo7O24.4H2O | 0,0088 g |
| KBr | 0,0119 g |
| KI | 0,0083 g |
| ZnSO4.7H2O | 0,0287 g |
| Cd(NO3)2.4H2O | 0,0154 g |
| Co(NO3)2.6H2O | 0,0146 g |
| CuSO4.5H2O | 0,0125 g |
| NiSO4(NH4)2SO4.6H2O | 0,0198 g |
| Cr(NO3)2.7H2O | 0,0037 g |
| V2O4(SO4)3.16H2O | 0,0035 g |
| Al2(SO4)3K2SO4.24 H2O | 0,0474 g |
Literature:
- Staub, R. (1961). Ernährungsphysiologisch-autökologische Untersuchungen an der planktischen Blaualge Oscillatoria rubescens DC. Schweizerische Zeitschrift für Hydrologie, 23, 82–198. DOI: 10.3929/ethz-a-00009-2335.